Expression of psychrophilic genes in mesophilic hosts: assessment of the folding state of a recombinant alpha-amylase.
نویسندگان
چکیده
Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 64 3 شماره
صفحات -
تاریخ انتشار 1998